The Zhao Bioinformatics Laboratory
pssRNAit: Designing Effective and Specific Plant RNAi siRNAs with Genome-wide Off-target Gene Assessment   
Location:  Analysis

 Please paste your sequence in FASTA format:    [Load demo data]     

 - Only accept one sequence every time
 - Uplimit of submission: 15K

 Please choose species name:      
cDNA/transcript libraries for selected species:
The designed siRNA candidates with the following motifs will be removed from final list.     
 1.  >2x(CAN) in antisense strand in Animals/Plants
 2.  AAAAA    in antisense strand in Animals/Plants
 3.  CCCCC    in antisense strand in Animals/Plants
 4.  GGGGG    in antisense strand in Animals/Plants
 5.  UUUUU    in antisense strand in Animals/Plants
 6.  UGUGU    in both      strand in Animals
 7.  GUCCUUCAA in both      strands in Animals
 8.  UGGC      in antisense strands in Animals
 9.  >2x(CUG)  in antisense strands in Animals
 10. >2x(CCG)  in antisense strands in Animals
 11. >2x(CGG)  in antisense strands in Animals
 12. WUAAAUW   in antisense strands in Animals


     
   Funding by the National Science Foundation    Funding by the Oklahoma Center for the Advancement of Science & Technology    Additional funding by the Samuel Roberts Noble Foundation

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