Please cite our paper: Mishra NK, Chang J, Zhao PX(2014). Prediction of membrane transport proteins and their substrate specificities using primary sequence information. PLoS One. 9(6):e100278..
TrSSP: the Transporter Substrate Specificity Prediction Server   
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Example of sequences in FASTA format:

>Seq1 optional comment
>Seq2 optional comment

Detailed description of the FASTA format is available at NCBI.

Important notes:
  • Empty lines are NOT accepted.
  • At present, the sequence input limit for first two (faster) classifiers is 2000 sequences.
  • Due to computational intense of PSI-BLAST searches against the Swissprot or Uniref90 databases and their corresponding conversion to PSSM matrices, the current limit for PSSM based and AAindex + PSSM based classifier are 1000 or 280 sequences per submission, respectively. For larger analysis, please contact us.
  • Each sequence should be at least more than 20 residues in length. The shorter sequence might be a "fragment" and so, the prediction will not make any sense.
Getting help:

     Patrick X. Zhao  


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