LegumeIP V3: From Models to Crops - An Integrative Gene Discovery Platform for Translational Genomics in Legumes

LegumeIP V3: From Models to Crops - An Integrative Gene Discovery Platform for Translational Genomics in Legumes

[O. sativa (gene)]
Detail of Experiment mapped on Oryza sativa Japonica Osativa323V7

Details

Name	Adaptation Versus Senescence In Rice: Early Signalling Events Play A Role In Inducing Specificity And Tolerance To Salinity
Description	Salinity tolerance is a complex trait and, despite many efforts to obtain rice plants resistant to salt, few results have been achieved since a deeper understanding of the tolerance mechanisms is still needed. We used imaging of photosynthetic parameters, ion analysis and transcriptomic approaches to unveil differences between two rice varieties differing in salt sensitivity. Moreover, we analysed H2O2 production in roots, using a fluorescent probe, and the ensuing gene regulation. Transcriptomic analyses conducted in tolerant plants supported the set-up of an adaptive program consisting of allocating sodium preferentially to roots, restricting it to the oldest leaves and activating regulatory mechanisms of photosynthesis in new leaves. As a consequence, plants resumed growth even under prolonged salt stress. By contrast, in the susceptible variety, RNA profiling unveiled a mis-targeted response, leading to senescence and cell death. In roots of tolerant plants, an increase in H2O2 was observed as early as 5 minutes after treatment. Consequently, the expression of genes involved in perception, signal transduction and response to salt were induced at earlier times when compared to susceptible plant roots. Our results demonstrate that a prompt H2O2 signalling in roots participates to a coordinated response resulting in adaptation instead of senescence in salt treated rice plants. Overall design: Samples were collected after 3 days of treatment with 100 mM NaCl. Roots and leaves were collected separately from untreated and treated plants. Each sample was made of 6 pooled plants. Three biological replicates were performed.
Contact	medcomp, Molecular Medicine, University of Padova
Xref	PRJNA430515
Reference	Formentin E et al . 2018; Formentin E et al . 2018;
Conditions	8
Species	Oryza sativa
Metadata export	.CSV .JSON

Analysis

Mapped Genomes	Osativa323V7
Expression Analysis	Expression Profile / Differential Expression / Co-expression

Conditions

Name	Genotype	Mutation	Stage	Tissue	Treatment	Level	Time	Samples	Xref	Download alignments
Baldo_treated_leaf	Japonica Group	Baldo	stage V2	leaf blade and sheath	100 mM NaCl			Baldo_treated_leaf_1 Baldo_treated_leaf_2 Baldo_treated_leaf_3	SRR6502097 SRR6502098 SRR6502099	.bam .bai .csi .bam .bai .csi .bam .bai .csi
Baldo_treated_roots	Japonica Group	Baldo	stage V2	roots	100 mM NaCl			Baldo_treated_roots_1 Baldo_treated_roots_2 Baldo_treated_roots_3	SRR6502103 SRR6502104 SRR6502105	.bam .bai .csi .bam .bai .csi .bam .bai .csi
Baldo_untreated_leaf	Japonica Group	Baldo	stage V2	leaf blade and sheath	none			Baldo_untreated_leaf_1 Baldo_untreated_leaf_2 Baldo_untreated_leaf_3	SRR6502100 SRR6502101 SRR6502102	.bam .bai .csi .bam .bai .csi .bam .bai .csi
Baldo_untreated_roots	Japonica Group	Baldo	stage V2	roots	none			Baldo_untreated_roots_1 Baldo_untreated_roots_2 Baldo_untreated_roots_3	SRR6502106 SRR6502107 SRR6502108	.bam .bai .csi .bam .bai .csi .bam .bai .csi
VialoneNano_treated_leaf	Japonica Group	Vialone Nano	stage V2	leaf blade and sheath	100 mM NaCl			VialoneNano_treated_leaf_1 VialoneNano_treated_leaf_2 VialoneNano_treated_leaf_3	SRR6502085 SRR6502086 SRR6502087	.bam .bai .csi .bam .bai .csi .bam .bai .csi
VialoneNano_treated_roots	Japonica Group	Vialone Nano	stage V2	roots	100 mM NaCl			VialoneNano_treated_roots_1 VialoneNano_treated_roots_2 VialoneNano_treated_roots_3	SRR6502091 SRR6502092 SRR6502093	.bam .bai .csi .bam .bai .csi .bam .bai .csi
VialoneNano_untreated_leaf	Japonica Group	Vialone Nano	stage V2	leaf blade and sheath	none			VialoneNano_untreated_leaf_1 VialoneNano_untreated_leaf_2 VialoneNano_untreated_leaf_3	SRR6502088 SRR6502089 SRR6502090	.bam .bai .csi .bam .bai .csi .bam .bai .csi
VialoneNano_untreated_roots	Japonica Group	Vialone Nano	stage V2	roots	none			VialoneNano_untreated_roots_1 VialoneNano_untreated_roots_2 VialoneNano_untreated_roots_3	SRR6502094 SRR6502095 SRR6502096	.bam .bai .csi .bam .bai .csi .bam .bai .csi