LegumeIP V3: From Models to Crops - An Integrative Gene Discovery Platform for Translational Genomics in Legumes

  • [H. vulgare (gene)]
  • Detail of Experiment mapped on Hordeum vulgare vulgare L Hvulgare462r1

Details

NameMODIFICATION OF BARLEY PLANT PRODUCTIVITY THROUGH REGULATION OF CYTOKININ CONTENT BY REVERSE-GENETICS APPROACHES
DescriptionBarley is one of the most important cereals, which is used for breweries, animal and human feeds. Genetic manipulation of plant hormone cytokinin (CK) levels may influence several physiological processes, besides others stress tolerance, root formation and crop yield. In planta, endogenous CK status is finely regulated by the enzyme cytokinin dehydrogenase (EC 1.5.99.12; CKX), that irreversibly cleaves the N6-side chain of adenine-derived CKs. Increasing grain yield by mean of manipulation of endogenous CK content was assayed by the silencing of the HvCKX1 gene. Moreover, to elucidate the putative role of HvCKX1 gene on grain production, knocked-out Hvckx1 mutant plants were generated using RNA-guided Cas9 system. Overall design: Examination of 2 independent transgenic lines with silenced HvCKX1 gene and control line in 2 developmental stages of barley grains. Each sample was analysed in 3 biological replicates
ContactLaboratory of experimental medicine, Palacky University
XrefPRJNA488274
Reference
Conditions6
SpeciesHordeum vulgare
Metadata export .CSV   .JSON

Analysis

Mapped Genomes Hvulgare462r1    
Expression Analysis Expression Profile /  Differential Expression /  Co-expression

Conditions

Name Genotype Mutation Stage Tissue Treatment Level Time Samples Xref Download alignments
CTRL_49 azygous control Golden Promise BBCH scale - stage 49 whole spike
  • CTRL_49_1
  • CTRL_49_2
  • CTRL_49_3
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CTRL_59 azygous control Golden Promise BBCH scale - stage 59 whole spike
  • CTRL_59_1
  • CTRL_59_2
  • CTRL_59_3
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KD-CKX1_17_10_49 KD-CKX1 line Golden Promise BBCH scale - stage 49 whole spike
  • KD-CKX1_17_10_49_1
  • KD-CKX1_17_10_49_2
  • KD-CKX1_17_10_49_3
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KD-CKX1_17_10_59 KD-CKX1 line Golden Promise BBCH scale - stage 59 whole spike
  • KD-CKX1_17_10_59_1
  • KD-CKX1_17_10_59_2
  • KD-CKX1_17_10_59_3
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KD-CKX1_5.8_49 KD-CKX1 line Golden Promise BBCH scale - stage 49 whole spike
  • KD-CKX1_5.8_49_1
  • KD-CKX1_5.8_49_2
  • KD-CKX1_5.8_49_3
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KD-CKX1_5.8_59 KD-CKX1 line Golden Promise BBCH scale - stage 59 whole spike
  • KD-CKX1_5.8_59_1
  • KD-CKX1_5.8_59_2
  • KD-CKX1_5.8_59_3
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