| Name | The tomato MYB21 acts in ovules to mediate jasmonate-regulated fertility |
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| Description | The function of the plant hormone jasmonic acid (JA) in development of tomato flowers was analyzed with a mutant defective in JA perception (jasmonate-insensitive1-1, jai1-1). In contrast to Arabidopsis JA-insensitive plants that are male sterile, the tomato mutant jai1-1 exhibits major defects in female development resulting in female sterility. To identify putative JA-dependent regulatory components, transcriptomics was performed using isolated ovules of three different stages of flower development, from both wild type and jai1-1. Among the strongly down-regulated genes in jai1-1, one encoding a MYB transcription factor (SlMYB21) was found. Its orthologue in Arabidopsis has a crucial role in JA-regulated stamen development. SlMYB21 showed transcription factor activity in yeast, interaction with SlJAZ9 in yeast and in planta, and complemented the Arabidopsis mutant myb21-5. To analyze SlMYB21 function in tomato ovule development, CRISPR/Cas9 mutants were created and a TILLING mutant was identified, all showing female sterility and therefore corroborating a function of MYB21 in tomato ovule development. Transcriptomics from wild type, jai1-1 and myb21-2 carpels revealed processes that might be controlled by SlMYB21. The data suggest a positive regulation of JA biosynthesis by SlMYB21, but a negative regulation of the action of auxin and GA. The results demonstrate that SlMYB21 mediates at least partially the action of JA and might control the flower to fruit transition. |
| Contact | Leibniz Institute of Plant Biochemistry Department of Cell and Metabolic Biology |
| Xref | PRJEB30489 |
| Reference |
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| Conditions | 3 |
| Species | Solanum lycopersicum |
| Metadata export | .CSV .JSON |
| Mapped Genomes | ITAG3.2 |
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| Expression Analysis | Expression Profile / Differential Expression / Co-expression |
| Name | Genotype | Mutation | Stage | Tissue | Treatment | Level | Time | Samples | Xref | Download alignments |
|---|---|---|---|---|---|---|---|---|---|---|
| cri | myb21-2 | flower development stage 3 | carpel |
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.bam
.bai
.csi
.bam .bai .csi .bam .bai .csi |
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| jai | jai1-1 | flower development stage 3 | carpel |
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.bam
.bai
.csi
.bam .bai .csi .bam .bai .csi |
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| wt | wild type genotype | flower development stage 3 | carpel |
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.bam
.bai
.csi
.bam .bai .csi .bam .bai .csi |