Name | Transcript Profiling of Common Bean Embryo Regions at Globular Stage Using Laser Capture Microdissection Coupled with RNA-Seq |
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Description | We used laser capture microdissection (LCM) to capture globular stage common bean embryo proper and suspensor, and profiled the transcriptome of these two embryo regions using next-generation sequencing. Our long-term goal is to understand the region-specific differentiation processes that occur during early embryo development and how genes are activated specifically in the suspensor and embryo proper. Overall design: Illumina sequencing of transcripts from laser-captured embryo proper and suspensor regions of common bean globular stage embryo. Two biological replicates were collected for each embryo region |
Contact | Molecular, Cell and Developmental Biology, University of California, Los Angeles |
Xref | PRJNA246611 |
Reference |
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Conditions | 2 |
Species | Phaseolus vulgaris |
Metadata export | .CSV .JSON |
Mapped Genomes | Pv442V2.1 |
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Expression Analysis | Expression Profile / Differential Expression / Co-expression |
Name | Genotype | Mutation | Stage | Tissue | Treatment | Level | Time | Samples | Xref | Download alignments |
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EmbryoProper | G19833 | Six days after pollination | globular-stage embryo | Laser-captured embryo proper region from globular-stage embryo |
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Suspensor | G19833 | Six days after pollination | globular-stage embryo | Laser-captured suspensor region from globular-stage embryo |
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