LegumeIP V3: From Models to Crops - An Integrative Gene Discovery Platform for Translational Genomics in Legumes

  • [P. vulgaris (gene)]
  • Detail of Experiment mapped on Phaseolus vulgaris Pv442V2.1

Details

NameTranscript Profiling of Common Bean Embryo Regions at Globular Stage Using Laser Capture Microdissection Coupled with RNA-Seq
DescriptionWe used laser capture microdissection (LCM) to capture globular stage common bean embryo proper and suspensor, and profiled the transcriptome of these two embryo regions using next-generation sequencing. Our long-term goal is to understand the region-specific differentiation processes that occur during early embryo development and how genes are activated specifically in the suspensor and embryo proper. Overall design: Illumina sequencing of transcripts from laser-captured embryo proper and suspensor regions of common bean globular stage embryo. Two biological replicates were collected for each embryo region
ContactMolecular, Cell and Developmental Biology, University of California, Los Angeles
XrefPRJNA246611
Reference
Conditions2
SpeciesPhaseolus vulgaris
Metadata export .CSV   .JSON

Analysis

Mapped Genomes Pv442V2.1    
Expression Analysis Expression Profile /  Differential Expression /  Co-expression

Conditions

Name Genotype Mutation Stage Tissue Treatment Level Time Samples Xref Download alignments
EmbryoProper G19833 Six days after pollination globular-stage embryo Laser-captured embryo proper region from globular-stage embryo
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  • EmbryoProper_8
  • EmbryoProper_9
  • EmbryoProper_10
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Suspensor G19833 Six days after pollination globular-stage embryo Laser-captured suspensor region from globular-stage embryo
  • Suspensor_1
  • Suspensor_2
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  • Suspensor_8
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