LegumeIP V3: From Models to Crops - An Integrative Gene Discovery Platform for Translational Genomics in Legumes

Name	TSAR1 and TSAR2 activate triterpene saponin production in Medicago truncatula
Description	We identified two novel transcription factors ‘Triterpene Saponin Activation Regulator’ (TSAR) 1 and 2 that each activate a specific branch of saponin synthesis in M. truncatula. Therefore we generated three independent TSAR1 and three independent TSAR2 overexpression (OE) hairy root lines. As a control we raised three independent hairy root lines expressing a non-functional GUS gene. By qRT-PCR analyses and metabolite profiling of these lines, we found that TSAR1 OE leads to accumulation of non-haemolytic saponins whereas TSAR2 instigates accumulation of haemolytic saponins. To identify additional targets of TSAR1 and TSAR2 we carried out a genome-wide transcript profiling study by RNA-Seq.
Contact	Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium Department of Plant Systems Biology,VIB, Gent, Belgium
Xref	PRJEB9179
Reference	Mertens J et al . 2016;
Conditions	3
Species	Medicago truncatula
Metadata export	.CSV .JSON

Mapped Genomes	MtrunA17r5.0 MtV4.0
Expression Analysis	Expression Profile / Differential Expression / Co-expression

Name	Genotype	Mutation	Stage	Tissue	Samples	Xref	Download alignments
CONTROL	Jemalong J5	CONTROL	21 days	Hairy root	CONTROL_1 CONTROL_2 CONTROL_3	ERR865495 ERR865500 ERR865498	.bam .bai .csi .bam .bai .csi .bam .bai .csi
TSAR1_OE	Jemalong J5	TSAR1 over expression	21 days	Hairy root	TSAR1_OE_1 TSAR1_OE_2 TSAR1_OE_3	ERR865497 ERR865493 ERR865492	.bam .bai .csi .bam .bai .csi .bam .bai .csi
TSAR2_OE	Jemalong J5	TSAR2 over expression	21 days	Hairy root	TSAR2_OE_1 TSAR2_OE_2 TSAR2_OE_3	ERR865494 ERR865496 ERR865499	.bam .bai .csi .bam .bai .csi .bam .bai .csi