LegumeIP V3: From Models to Crops - An Integrative Gene Discovery Platform for Translational Genomics in Legumes

  • [M. truncatula (gene)]
  • Detail of Experiment mapped on Medicago truncatula A17 MtV4.0

Details

Nameimportance of DNA (de)methylation and epigenetic regulations for indeterminate nodule development
DescriptionEpigenetic mechanisms are known to regulate gene expression but no regulators have been characterized in the context of nodule development in legumes. Here, we describe DEMETER (DME) in Medicago truncatula, a gene coding for a demethylase that is required for normal symbiosome differentiation during nodule development. MtDMEis strongly upregulated in indeterminate nodules in the differentiation zone. The role of DMEduring nodule development was analyzed by using an RNA interference approach. This study showed that DMEis essential for the production of functional nitrogen fixing nodules which present improperly differentiated bacteroids. Transcriptome analysis of RNAi plants showed that MtDMEis involved in the expression of 1166 genes correlated with nodule differentiation genes such as NCR genes.Our work thus demonstrates the importance of DNA (de)methylation and epigenetic regulations for indeterminate nodule development.
ContactINRA-CNRS
XrefPRJNA301237
Reference
Conditions5
SpeciesMedicago truncatula
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Analysis

Mapped Genomes MtrunA17r5.0     MtV4.0    
Expression Analysis Expression Profile /  Differential Expression /  Co-expression

Conditions

Name Genotype Mutation Stage Tissue Treatment Level Time Samples Xref Download alignments
efd_10dpi Jemalong A17 root nodule Inoculated with S. meliloti 10dpi
  • efd_10dpi_1
  • efd_10dpi_2
  • efd_10dpi_3
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pDME:DMEi_28dpi Jemalong A17 root nodule Inoculated with S. meliloti 28dpi
  • pDME:DMEi_28dpi_1
  • pDME:DMEi_28dpi_2
  • pDME:DMEi_28dpi_3
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pDME:DMEi_9dpi Jemalong A17 root nodule Inoculated with S. meliloti 9dpi
  • pDME:DMEi_9dpi_1
  • pDME:DMEi_9dpi_2
  • pDME:DMEi_9dpi_3
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pDME:GUS_28dpi Jemalong A17 root nodule Inoculated with S. meliloti 28dpi
  • pDME:GUS_28dpi_1
  • pDME:GUS_28dpi_2
  • pDME:GUS_28dpi_3
 .bam    .bai    .csi   
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pDME:GUS_9dpi Jemalong A17 root nodule Inoculated with S. meliloti 9dpi
  • pDME:GUS_9dpi_1
  • pDME:GUS_9dpi_2
  • pDME:GUS_9dpi_3
 .bam    .bai    .csi   
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.bam    .bai    .csi