LegumeIP V3: From Models to Crops - An Integrative Gene Discovery Platform for Translational Genomics in Legumes

  • [L. angustifolius (gene)]
  • Detail of Experiment mapped on Lupinus angustifolius Tanjil LaV1

Details

NameAnalysis of conglutin seed storage proteins across lupin species using transcriptomic, protein and comparative genomic approaches.
DescriptionAn in-depth transcriptome and draft genome from the narrow-leafed lupin (NLL; Lupinus angustifolius) variety, Tanjil, were examined and 16 conglutin genes were identified. Using RNAseq data sets, the structure and expression of these 16 conglutin genes were analysed across eight lupin varieties from five lupin species. Phylogenic analysis suggest that the α and γ conglutins diverged prior to lupin speciation while β and δ members diverged both prior and after speciation. A comparison of the expression of the 16 conglutin genes was performed, and in general the conglutin genes showed similar levels of RNA expression among varieties within species, but quite distinct expression patterns between lupin species. Antibodies were generated against the specific conglutin families and immunoblot analyses were used to compare the levels of conglutin proteins in various tissues and during different stages of seed development in NLL, Tanjil, confirming the expression in the seed. This analysis showed that the conglutins were expressed highly at the mature seed stage, in all lupin species, and a range of polypeptide sizes were observed for each conglutin family.
ContactCSIRO
XrefPRJNA271721
Reference
Conditions6
SpeciesLupinus angustifolius
Metadata export .CSV   .JSON

Analysis

Mapped Genomes LaV1    
Expression Analysis Expression Profile /  Differential Expression /  Co-expression

Conditions

Name Genotype Mutation Stage Tissue Treatment Level Time Samples Xref Download alignments
angustifolius_p27255 P27255 20-26 days after anthesis seed growth at 22/18 degrees Celsius over a 14/10 hours day/night schedule, under 600 Photosynthetically Active Radiation micromol/m/s lights
  • angustifolius_p27255_1
 .bam    .bai    .csi   
angustifolius_p27255_SE P27255 20-26 days after anthesis seed growth at 22/18 degrees Celsius over a 14/10 hours day/night schedule, under 600 Photosynthetically Active Radiation micromol/m/s lights
  • angustifolius_p27255_SE_1
 .bam    .bai    .csi   
angustifolius_tanjil Tanjil 20-26 days after anthesis seed growth at 22/18 degrees Celsius over a 14/10 hours day/night schedule, under 600 Photosynthetically Active Radiation micromol/m/s lights
  • angustifolius_tanjil_1
 .bam    .bai    .csi   
angustifolius_tanjil_SE Tanjil 20-26 days after anthesis seed growth at 22/18 degrees Celsius over a 14/10 hours day/night schedule, under 600 Photosynthetically Active Radiation micromol/m/s lights
  • angustifolius_tanjil_SE_1
 .bam    .bai    .csi   
angustifolius_unicrop Unicrop 20-26 days after anthesis seed growth at 22/18 degrees Celsius over a 14/10 hours day/night schedule, under 600 Photosynthetically Active Radiation micromol/m/s lights
  • angustifolius_unicrop_1
 .bam    .bai    .csi   
angustifolius_unicrop_SE Unicrop 20-26 days after anthesis seed growth at 22/18 degrees Celsius over a 14/10 hours day/night schedule, under 600 Photosynthetically Active Radiation micromol/m/s lights
  • angustifolius_unicrop_SE_1
 .bam    .bai    .csi