| Name | Comparative analysis of drought-responsive transcriptome in soybean lines contrasting for canopy wilting |
|---|---|
| Description | Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of abiotic stress molecular pathways. The goals of this study are to compare NGS-derived transcriptome profiling (RNA-seq) of contrasting slow wilting lines to quantify transcript abumdance under drought stress condition Overall design: Methods: The three biological replicates of DS line, Pana (control and drought samples) and DT line, PI 567690 (control and drought samples) leaf sample RNA were multiplexed and sequenced on an Illumina Hi-Seq 2000 platform. The RNA concentration of each sample was approximately 200ng/µl with a quantity of 50 µl.isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays |
| Contact | Digital Biology Lab, Computer Science, University of Missouri |
| Xref | PRJNA288296 |
| Reference | |
| Conditions | 4 |
| Species | Glycine max |
| Metadata export | .CSV .JSON |
| Mapped Genomes | Gm275Wm82a2V1 Gm508Wm82a4V1 |
|---|---|
| Expression Analysis | Expression Profile / Differential Expression / Co-expression |
| Name | Genotype | Mutation | Stage | Tissue | Treatment | Level | Time | Samples | Xref | Download alignments |
|---|---|---|---|---|---|---|---|---|---|---|
| DS_control | Drought Susceptible | R2 stage | leaf | Control condition |
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| DS_drought15d | Drought Susceptible | R2 stage | leaf | Drought stressed for 15 days |
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| DT_control | Drought Tolerant | R2 stage | leaf | Control condition |
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| DT_drought15d | Drought Tolerant | R2 stage | leaf | Drought stressed for 15 days |
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.bam .bai .csi .bam .bai .csi |