LegumeIP V3: From Models to Crops - An Integrative Gene Discovery Platform for Translational Genomics in Legumes

  • [G. max (gene)]
  • Detail of Experiment mapped on Glycine max Wm82 Gm275Wm82a2V1

Details

NameWhole genome-wide transcript profiling to identify differentially expressed genes associated with seed field emergence in two soybean low phytate mutants
DescriptionSeed germination is important to soybean (Glycine max) growth and development, ultimately affecting soybean yield. A lower seed field emergence has been the main hindrance for breeding soybeans low in phytate. Although this reduction could be overcome by additional breeding and selection, the mechanisms of seed germination in different low phytate mutants remain unknown. In this study, we performed a comparative transcript analysis of two low phytate soybean mutants (TW-1 and TW-1-M), which have the same mutation, a 2 bp deletion in GmMIPS1, but show a significant difference in seed field emergence. Overall design: 18 samples were analyzed in this research, and three replicates were included.
ContactZhejiang Academy of Agricultural Sciences
XrefPRJNA325298
Reference
Conditions6
SpeciesGlycine max
Metadata export .CSV   .JSON

Analysis

Mapped Genomes Gm275Wm82a2V1     Gm508Wm82a4V1    
Expression Analysis Expression Profile /  Differential Expression /  Co-expression

Conditions

Name Genotype Mutation Stage Tissue Treatment Level Time Samples Xref Download alignments
TW-1_veryLowRate_12hSeedsImbibition low phytate soybean mutant TW-1 germination of soybean seed seed very low rate 12h after the seeds finished imbibition
  • TW-1_veryLowRate_12hSeedsImbibition_1
  • TW-1_veryLowRate_12hSeedsImbibition_2
  • TW-1_veryLowRate_12hSeedsImbibition_3
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TW-1_veryLowRate_emergingPrimaryRoot1mm low phytate soybean mutant TW-1 germination of soybean seed seed very low rate emerging primary root reached 1mm in length
  • TW-1_veryLowRate_emergingPrimaryRoot1mm_1
  • TW-1_veryLowRate_emergingPrimaryRoot1mm_2
  • TW-1_veryLowRate_emergingPrimaryRoot1mm_3
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TW-1_veryLowRate_imbibitionStage low phytate soybean mutant TW-1 germination of soybean seed seed very low rate imbibition stage
  • TW-1_veryLowRate_imbibitionStage_1
  • TW-1_veryLowRate_imbibitionStage_2
  • TW-1_veryLowRate_imbibitionStage_3
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TW-1-M_higherRate_12hSeedsImbibition low phytate soybean mutant TW-1-M germination of soybean seed seed significantly higher rate compared to TW-1 12h after the seeds finished imbibition
  • TW-1-M_higherRate_12hSeedsImbibition_1
  • TW-1-M_higherRate_12hSeedsImbibition_2
  • TW-1-M_higherRate_12hSeedsImbibition_3
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TW-1-M_higherRate_emergingPrimaryRoot1mm low phytate soybean mutant TW-1-M germination of soybean seed seed significantly higher rate compared to TW-1 emerging primary root reached 1mm in length
  • TW-1-M_higherRate_emergingPrimaryRoot1mm_1
  • TW-1-M_higherRate_emergingPrimaryRoot1mm_2
  • TW-1-M_higherRate_emergingPrimaryRoot1mm_3
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TW-1-M_higherRate_imbibitionStage low phytate soybean mutant TW-1-M germination of soybean seed seed significantly higher rate compared to TW-1 imbibition stage
  • TW-1-M_higherRate_imbibitionStage_1
  • TW-1-M_higherRate_imbibitionStage_2
  • TW-1-M_higherRate_imbibitionStage_3
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