LegumeIP V3: From Models to Crops - An Integrative Gene Discovery Platform for Translational Genomics in Legumes

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Name Experiment Genotype Mutation Stage Tissue/organ Treatment Level Time replicates
Control Transcriptomic response t ...... wild type A17 5 days Root Hairs control 0h 2
NodFactor_20h Transcriptomic response t ...... wild type A17 5 days Root Hairs Nod Factor 20h 2
NodFactor_4h Transcriptomic response t ...... wild type A17 5 days Root Hairs Nod Factor 4h 2
K-1000xK-Root Macronutrient Deficiencie ...... A17 21 days after germination root Low Potassium 2.45 µM K 21 days 3
K-1000xK-Shoot Macronutrient Deficiencie ...... A17 21 days after germination shoot Low Potassium 2.45 µM K 21 days 3
K-FN-Root Macronutrient Deficiencie ...... A17 21 days after germination root Full Nutrition Full Potassium (2.45 mM K) 21 days 3
K-FN-Shoot Macronutrient Deficiencie ...... A17 21 days after germination shoot Full Nutrition Full Potassium (2.45 mM K) 21 days 3
K-Re-supply-Root Macronutrient Deficiencie ...... A17 21 days after germination root Low Potassium;
K Re-supply 		2.45 µM K;
2.45 mM K 		21 days;
4x 1.5 Hours 		3
K-Re-supply-Shoot Macronutrient Deficiencie ...... A17 21 days after germination shoot Low Potassium;
K Re-supply 		2.45 µM K;
2.45 mM K 		21 days;
4x 1.5 Hours 		3
N-10xN-Root Macronutrient Deficiencie ...... A17 21 days after germination root Low Nitrogen 0.2 mM N 21 days 3
N-10xN-Shoot Macronutrient Deficiencie ...... A17 21 days after germination shoot Low Nitrogen 0.2 mM N 21 days 3
N-FN-Root Macronutrient Deficiencie ...... A17 21 days after germination root Full Nutrition Full Nitrogen (2.0 mM N) 21 days 3
N-FN-Shoot Macronutrient Deficiencie ...... A17 21 days after germination shoot Full Nutrition Full Nitrogen (2.0 mM N) 21 days 3
N-Re-supply-Root Macronutrient Deficiencie ...... A17 21 days after germination root Low Nitrogen;
N Re-supply 		0.2 mM N;
2.0 mM N 		21 days;
4x 1.5 Hours 		3
N-Re-supply-Shoot Macronutrient Deficiencie ...... A17 21 days after germination shoot Low Nitrogen;
N Re-supply 		0.2 mM N;
2.0 mM N 		21 days;
4x 1.5 Hours 		3
P-100xP-Root Macronutrient Deficiencie ...... A17 21 days after germination root Low Phosphorous 6.0 µM P 21 days 3
P-100xP-Shoot Macronutrient Deficiencie ...... A17 21 days after germination shoot Low Phosphorous 6.0 µM P 21 days 3
P-FN-Root Macronutrient Deficiencie ...... A17 21 days after germination root Full Nutrition Full Phosphorous (0.6 mM P); Full Sulfur (0.6 mM S) 21 days 3
P-FN-Shoot Macronutrient Deficiencie ...... A17 21 days after germination shoot Full Nutrition Full Phosphorous (0.6 mM P); Full Sulfur (0.6 mM S) 21 days 3
P-Re-supply-Root Macronutrient Deficiencie ...... A17 21 days after germination root Low Phosphorous;
P Re-supply 		6.0 µM P;
0.6 mM P 		21 days;
4x 1.5 Hours 		3
P-Re-supply-Shoot Macronutrient Deficiencie ...... A17 21 days after germination shoot Low Phosphorous;
P Re-supply 		6.0 µM P;
0.6 mM P 		21 days;
4x 1.5 Hours 		3
S-100xS-Root Macronutrient Deficiencie ...... A17 21 days after germination root Low Sulfur 6.0 µM S 21 days 3
S-100xS-Shoot Macronutrient Deficiencie ...... A17 21 days after germination shoot Low Sulfur 6.0 µM S 21 days 3
S-FN-Root Macronutrient Deficiencie ...... A17 21 days after germination root Full Nutrition Full Phosphorous (0.6 mM P); Full Sulfur (0.6 mM S) 21 days 3
S-FN-Shoot Macronutrient Deficiencie ...... A17 21 days after germination shoot Full Nutrition Full Phosphorous (0.6 mM P); Full Sulfur (0.6 mM S) 21 days 3
S-Re-supply-Root Macronutrient Deficiencie ...... A17 21 days after germination root Low Sulfur;
S Re-supply 		6.0 µM S;
0.6 mM S 		21 days;
4x 1.5 Hours 		3
S-Re-supply-Shoot Macronutrient Deficiencie ...... A17 21 days after germination shoot Low Sulfur;
S Re-supply 		6.0 µM S;
0.6 mM S 		21 days;
4x 1.5 Hours 		3
Pooja-FN_Shoot Macronutrient Deficiencie ...... A17 22 days after germination shoot Full Nutrition 0.6 mM P; 1.2 mM N beginning at 14 days 2
Pooja-FN-Root Macronutrient Deficiencie ...... A17 22 days after germination root Full Nutrition 0.6 mM P; 1.2 mM N beginning at 14 days 3
Pooja-NoN_Root Macronutrient Deficiencie ...... A17 22 days after germination root No Nitrogen 0 µM N beginning at 14 days 3