LegumeIP V3: From Models to Crops - An Integrative Gene Discovery Platform for Translational Genomics in Legumes

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Name Experiment Genotype Mutation Stage Tissue/organ Treatment Level Time replicates
Control transcriptome profiling o ...... R108 4th trifolate leaf of 3 week old plant leaf Infiltrated with 35S:MtCOla 4 days post infiltration 3
LAP1 transcriptome profiling o ...... R108 4th trifolate leaf of 3 week old plant leaf Infiltrated with 35S:MtLAP1 4 days post infiltration 3
Mtbud Plant Organs A17 5 weeks after transplanting bud 3
Mtlargepod Plant Organs A17 Fully grown green and yellow pods containing seeds harvested at 8 - 10 weeks fruit 3
Mtleafblade Plant Organs A17 5 weeks after transplanting leaf 3
Mtmedpod Plant Organs A17 Medium-sized Pods containing seeds harvested at 8 - 10 weeks fruit 3
Mtopenflower Plant Organs A17 Fully opened flower at 6-7 weeks flower 3
Mtpetiole Plant Organs A17 5 weeks after transplanting petiole 3
Mtroot Plant Organs A17 5 weeks after transplanting root 3
Mtsmallpod Plant Organs A17 Pods containing seeds just forming at 8-10 weeks fruit 3
Mtstem Plant Organs A17 5 weeks after transplanting stalk 3
Mt.nodule.T0 Transcriptomic changes in ...... Jemalong A17 8-week-old root nodule Inoculated with S. meliloti;
well-watered control nodules (T0) 		2
Mt.nodule.T2 Transcriptomic changes in ...... Jemalong A17 8-week-old root nodule Inoculated with S. meliloti;
drought for two (T2) days 		2
Mt.nodule.T4 Transcriptomic changes in ...... Jemalong A17 8-week-old root nodule Inoculated with S. meliloti;
drought for four (T4) days 		2
Mt.root.T0 Transcriptomic changes in ...... Jemalong A17 8-week-old root Medicago truncatula non-inoculated roots;
well-watered control nodules (T0) 		2
MtSm-RbmL-IZ Nodule Development 2 A17 22 days after germination + 15 days post inoculation root nodule ammonium nitrate supplemented;
Inoculation with the S. meliloti 2011 strain GMI11495 (4 days in N free prior to inoculation) 		10 m MNH4NO3 18 days;
22 days 		3
MtSm-RbmL-ZI Nodule Development 2 A17 22 days after germination + 15 days post inoculation root nodule ammonium nitrate supplemented;
Inoculation with the S. meliloti 2011 strain GMI11495 (4 days in N free prior to inoculation) 		10 m MNH4NO3 18 days;
22 days 		3
MtSm-RbmL-ZIId Nodule Development 2 A17 22 days after germination + 15 days post inoculation root nodule ammonium nitrate supplemented;
Inoculation with the S. meliloti 2011 strain GMI11495 (4 days in N free prior to inoculation) 		10 m MNH4NO3 18 days;
22 days 		3
MtSm-RbmL-ZIII Nodule Development 2 A17 22 days after germination + 15 days post inoculation root nodule ammonium nitrate supplemented;
Inoculation with the S. meliloti 2011 strain GMI11495 (4 days in N free prior to inoculation) 		10 m MNH4NO3 18 days;
22 days 		3
MtSm-RbmL-ZIIp Nodule Development 2 A17 22 days after germination + 15 days post inoculation root nodule ammonium nitrate supplemented;
Inoculation with the S. meliloti 2011 strain GMI11495 (4 days in N free prior to inoculation) 		10 m MNH4NO3 18 days;
22 days 		3