LegumeIP V3: From Models to Crops - An Integrative Gene Discovery Platform for Translational Genomics in Legumes

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Name Experiment Genotype Mutation Stage Tissue/organ Treatment Level Time replicates
Control transcriptome profiling o ...... R108 4th trifolate leaf of 3 week old plant leaf Infiltrated with 35S:MtCOla 4 days post infiltration 3
LAP1 transcriptome profiling o ...... R108 4th trifolate leaf of 3 week old plant leaf Infiltrated with 35S:MtLAP1 4 days post infiltration 3
BAP3hr Hormone treatment A17 3 days after germination root Cytokinin - 6-Benzylaminopurine;
Medicago seedlings Roots 		100 nM 6-Benzylaminopurine 3 hr 3
Control3hr Hormone treatment A17 3 days after germination root DMSO Control;
Medicago seedlings Roots 		DMSO Control 3 hr 3
IAA3hr Hormone treatment A17 3 days after germination root Auxin - Indole-3-acetic acid;
Medicago seedlings Roots 		1 uM Indole-3-acetic acid 3 hr 3
Pooja-FN_Shoot Macronutrient Deficiencie ...... A17 22 days after germination shoot Full Nutrition 0.6 mM P; 1.2 mM N beginning at 14 days 2
Pooja-FN-Root Macronutrient Deficiencie ...... A17 22 days after germination root Full Nutrition 0.6 mM P; 1.2 mM N beginning at 14 days 3
Pooja-NoN_Root Macronutrient Deficiencie ...... A17 22 days after germination root No Nitrogen 0 µM N beginning at 14 days 3
Pooja-NoN_Shoot Macronutrient Deficiencie ...... A17 22 days after germination shoot No Nitrogen 0 µM N beginning at 14 days 2
Pooja-NoP_Root Macronutrient Deficiencie ...... A17 22 days after germination root No Phosphorous 0 µM P beginning at 14 days 3
Pooja-NoP_Shoot Macronutrient Deficiencie ...... A17 22 days after germination shoot No Phosphorous 0 µM P beginning at 14 days 2
Mtbud Plant Organs A17 5 weeks after transplanting bud 3
Mtlargepod Plant Organs A17 Fully grown green and yellow pods containing seeds harvested at 8 - 10 weeks fruit 3
Mtleafblade Plant Organs A17 5 weeks after transplanting leaf 3
Mtmedpod Plant Organs A17 Medium-sized Pods containing seeds harvested at 8 - 10 weeks fruit 3
Mtopenflower Plant Organs A17 Fully opened flower at 6-7 weeks flower 3
Mtpetiole Plant Organs A17 5 weeks after transplanting petiole 3
Mtroot Plant Organs A17 5 weeks after transplanting root 3
Mtsmallpod Plant Organs A17 Pods containing seeds just forming at 8-10 weeks fruit 3
Mtstem Plant Organs A17 5 weeks after transplanting stalk 3