LegumeIP V3: From Models to Crops - An Integrative Gene Discovery Platform for Translational Genomics in Legumes

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Name Experiment Genotype Mutation Stage Tissue/organ Treatment Level Time replicates
CONTROL TSAR1 and TSAR2 activate ...... Jemalong J5 CONTROL 21 days Hairy root 3
TSAR1_OE TSAR1 and TSAR2 activate ...... Jemalong J5 TSAR1 over expression 21 days Hairy root 3
TSAR2_OE TSAR1 and TSAR2 activate ...... Jemalong J5 TSAR2 over expression 21 days Hairy root 3
A17_LCO_WT- CK dependent Rhizobium LC ...... A17 wild type genotype Susceptible zone E-MTAB-3007:A17 LCO WT002- 3h 3
A17_LCO_WT+ CK dependent Rhizobium LC ...... A17 wild type genotype Susceptible zone E-MTAB-3007:A17 LCO WT002+ 3h 3
A17_noLCO_WT- CK dependent Rhizobium LC ...... A17 wild type genotype Susceptible zone E-MTAB-3007:A17 noLCO WT003- 3h 3
A17_noLCO_WT+ CK dependent Rhizobium LC ...... A17 wild type genotype Susceptible zone E-MTAB-3007:A17 noLCO WT001+ 3h 3
Mtcre1_LCO_WT- CK dependent Rhizobium LC ...... A17 Mtcre1 mutant Susceptible zone E-MTAB-3007:Mtcre1 LCO WT003- 3h 3
Mtcre1_LCO_WT+ CK dependent Rhizobium LC ...... A17 Mtcre1 mutant Susceptible zone E-MTAB-3007:Mtcre1 LCO WT003+ 3h 3
Mtcre1_noLCO_WT- CK dependent Rhizobium LC ...... A17 Mtcre1 mutant Susceptible zone E-MTAB-3007:Mtcre1 noLCO WT001- 3h 3
Mtcre1_noLCO_WT+ CK dependent Rhizobium LC ...... A17 Mtcre1 mutant Susceptible zone E-MTAB-3007:Mtcre1 noLCO WT002+ 3h 3
line_2A Expression analysis of Mt ...... line 2A 3
Mt_ctrl Expression analysis of Mt ...... control 3
BAP3hr Hormone treatment A17 3 days after germination root Cytokinin - 6-Benzylaminopurine;
Medicago seedlings Roots 		100 nM 6-Benzylaminopurine 3 hr 3
Control3hr Hormone treatment A17 3 days after germination root DMSO Control;
Medicago seedlings Roots 		DMSO Control 3 hr 3
IAA3hr Hormone treatment A17 3 days after germination root Auxin - Indole-3-acetic acid;
Medicago seedlings Roots 		1 uM Indole-3-acetic acid 3 hr 3
Mt.nodule.T0 Transcriptomic changes in ...... Jemalong A17 8-week-old root nodule Inoculated with S. meliloti;
well-watered control nodules (T0) 		2
Mt.nodule.T2 Transcriptomic changes in ...... Jemalong A17 8-week-old root nodule Inoculated with S. meliloti;
drought for two (T2) days 		2
Mt.nodule.T4 Transcriptomic changes in ...... Jemalong A17 8-week-old root nodule Inoculated with S. meliloti;
drought for four (T4) days 		2
Mt.root.T0 Transcriptomic changes in ...... Jemalong A17 8-week-old root Medicago truncatula non-inoculated roots;
well-watered control nodules (T0) 		2
MtSm-RbmL-IZ Nodule Development 2 A17 22 days after germination + 15 days post inoculation root nodule ammonium nitrate supplemented;
Inoculation with the S. meliloti 2011 strain GMI11495 (4 days in N free prior to inoculation) 		10 m MNH4NO3 18 days;
22 days 		3
MtSm-RbmL-ZI Nodule Development 2 A17 22 days after germination + 15 days post inoculation root nodule ammonium nitrate supplemented;
Inoculation with the S. meliloti 2011 strain GMI11495 (4 days in N free prior to inoculation) 		10 m MNH4NO3 18 days;
22 days 		3
MtSm-RbmL-ZIId Nodule Development 2 A17 22 days after germination + 15 days post inoculation root nodule ammonium nitrate supplemented;
Inoculation with the S. meliloti 2011 strain GMI11495 (4 days in N free prior to inoculation) 		10 m MNH4NO3 18 days;
22 days 		3
MtSm-RbmL-ZIII Nodule Development 2 A17 22 days after germination + 15 days post inoculation root nodule ammonium nitrate supplemented;
Inoculation with the S. meliloti 2011 strain GMI11495 (4 days in N free prior to inoculation) 		10 m MNH4NO3 18 days;
22 days 		3
MtSm-RbmL-ZIIp Nodule Development 2 A17 22 days after germination + 15 days post inoculation root nodule ammonium nitrate supplemented;
Inoculation with the S. meliloti 2011 strain GMI11495 (4 days in N free prior to inoculation) 		10 m MNH4NO3 18 days;
22 days 		3