LegumeIP V3: From Models to Crops - An Integrative Gene Discovery Platform for Translational Genomics in Legumes

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Name Experiment Genotype Mutation Stage Tissue/organ Treatment Level Time replicates
AM-K RNA-seq of mycorrhizal Me ...... Jemalong A17 root Rhizophagus irregularis;
AM-K 		3
AM+K RNA-seq of mycorrhizal Me ...... Jemalong A17 root Rhizophagus irregularis;
AM+K 		3
NM-K RNA-seq of mycorrhizal Me ...... Jemalong A17 root control;
NM-K 		4
NM+K RNA-seq of mycorrhizal Me ...... Jemalong A17 root control;
NM+K 		4
Mt.nodule.T2 Transcriptomic changes in ...... Jemalong A17 8-week-old root nodule Inoculated with S. meliloti;
drought for two (T2) days 		2
Mt.nodule.T4 Transcriptomic changes in ...... Jemalong A17 8-week-old root nodule Inoculated with S. meliloti;
drought for four (T4) days 		2
Mt.root.T0 Transcriptomic changes in ...... Jemalong A17 8-week-old root Medicago truncatula non-inoculated roots;
well-watered control nodules (T0) 		2
Mt.nodule.T0 Transcriptomic changes in ...... Jemalong A17 8-week-old root nodule Inoculated with S. meliloti;
well-watered control nodules (T0) 		2
Salt_Root_No_HM198 Salt Stress HM198 25 days old root No Salt 3
Salt_Root_No_HM309 Salt Stress HM309 25 days old root No Salt 3
Salt_Root_Salt_HM198 Salt Stress HM198 25 days old root Salt - NaCl day 10 = 0.5% NaCl; day 15 = 1% NaCl; day 20 = 1.5% NaCl 3
Salt_Root_Salt_HM309 Salt Stress HM309 25 days old root Salt - NaCl day 10 = 0.5% NaCl; day 15 = 1% NaCl; day 20 = 1.5% NaCl 3
Salt_Shoot_No_HM198 Salt Stress HM198 25 days old shoot No Salt 3
Salt_Shoot_No_HM309 Salt Stress HM309 25 days old shoot No Salt 3
Salt_Shoot_Salt_HM198 Salt Stress HM198 25 days old shoot Salt - NaCl day 10 = 0.5% NaCl; day 15 = 1% NaCl; day 20 = 1.5% NaCl 3
Salt_Shoot_Salt_HM309 Salt Stress HM309 25 days old shoot Salt - NaCl day 10 = 0.5% NaCl; day 15 = 1% NaCl; day 20 = 1.5% NaCl 3
SUNN_Control_Root Symbiotic Interactions 2 A17 SUNN 18 days after planting root Whole root from un-colonized A17 sunn mutant Sterile soil At planting 3
SUNN_Control_Shoot Symbiotic Interactions 2 A17 SUNN 18 days after planting shoot Whole shoot from un-colonized A17 sunn mutant Sterile soil At planting 3
SUNN_Mycorrhiza_Root Symbiotic Interactions 2 A17 SUNN 18 days after planting root Whole root from mycorrhiza colonized A17 sunn mutant 10 % (w/w) soil inoculum At planting 3
SUNN_Mycorrhiza_Shoot Symbiotic Interactions 2 A17 SUNN 18 days after planting shoot Whole shoot from mycorrhiza colonized A17 sunn mutant 10 % (w/w) soil inoculum At planting 3
Wildtype_Control_Root Symbiotic Interactions 2 A17 None 18 days after planting root Whole root from un-colonized A17 wild type Sterile soil At planting 3
Wildtype_Control_Shoot Symbiotic Interactions 2 A17 None 18 days after planting shoot Whole shoot from un-colonized A17 wild type Sterile soil At planting 3
Wildtype_Mycorrhiza_Root Symbiotic Interactions 2 A17 None 18 days after planting root Whole root from mycorrhiza colonized A17 wild type 10 % (w/w) soil inoculum At planting 3
Wildtype_Mycorrhiza_Shoot Symbiotic Interactions 2 A17 None 18 days after planting shoot Whole shoot from mycorrhiza colonized A17 wild type 10 % (w/w) soil inoculum At planting 3
MtSm-RbmL-IZ Nodule Development 2 A17 22 days after germination + 15 days post inoculation root nodule ammonium nitrate supplemented;
Inoculation with the S. meliloti 2011 strain GMI11495 (4 days in N free prior to inoculation) 		10 m MNH4NO3 18 days;
22 days 		3
MtSm-RbmL-ZI Nodule Development 2 A17 22 days after germination + 15 days post inoculation root nodule ammonium nitrate supplemented;
Inoculation with the S. meliloti 2011 strain GMI11495 (4 days in N free prior to inoculation) 		10 m MNH4NO3 18 days;
22 days 		3
MtSm-RbmL-ZIId Nodule Development 2 A17 22 days after germination + 15 days post inoculation root nodule ammonium nitrate supplemented;
Inoculation with the S. meliloti 2011 strain GMI11495 (4 days in N free prior to inoculation) 		10 m MNH4NO3 18 days;
22 days 		3
MtSm-RbmL-ZIII Nodule Development 2 A17 22 days after germination + 15 days post inoculation root nodule ammonium nitrate supplemented;
Inoculation with the S. meliloti 2011 strain GMI11495 (4 days in N free prior to inoculation) 		10 m MNH4NO3 18 days;
22 days 		3
MtSm-RbmL-ZIIp Nodule Development 2 A17 22 days after germination + 15 days post inoculation root nodule ammonium nitrate supplemented;
Inoculation with the S. meliloti 2011 strain GMI11495 (4 days in N free prior to inoculation) 		10 m MNH4NO3 18 days;
22 days 		3