LegumeIP V3: From Models to Crops - An Integrative Gene Discovery Platform for Translational Genomics in Legumes

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Name Experiment Genotype Mutation Stage Tissue/organ Treatment Level Time replicates
A17_Mocktreated_2dpi important ethylene signal ...... A17 A17 (wild type) Seedling root Mock treated 2 days 3
A17_Mocktreated_7dpi important ethylene signal ...... A17 A17 (wild type) Seedling root Mock treated 7 days 3
A17_R.solani_inoculated_treated_2dpi important ethylene signal ...... A17 A17 (wild type) Seedling root R. solani inoculated treated 2 days 3
A17_R.solani_inoculated_treated_7dpi important ethylene signal ...... A17 A17 (wild type) Seedling root R. solani inoculated treated 7 days 3
sickle_Mocktreated_2dpi important ethylene signal ...... A17 sickle mutant (ethylene insensitive, point mutation in A17) Seedling root Mock treated 2 days 3
sickle_Mocktreated_7dpi important ethylene signal ...... A17 sickle mutant (ethylene insensitive, point mutation in A17) Seedling root Mock treated 7 days 3
sickle_R.solani_inoculated_treated_2dpi important ethylene signal ...... A17 sickle mutant (ethylene insensitive, point mutation in A17) Seedling root R. solani inoculated treated 2 days 3
sickle_R.solani_inoculated_treated_7dpi important ethylene signal ...... A17 sickle mutant (ethylene insensitive, point mutation in A17) Seedling root R. solani inoculated treated 7 days 3
M-R- effects of rhizobia and m ...... JA17 7 weeks roots no mycorrhizal fungi or rhizobia 8
M-R+ effects of rhizobia and m ...... JA17 7 weeks roots no mycorrhizal fungi but rhizobia present 8
M+R- effects of rhizobia and m ...... JA17 7 weeks roots mycorrhizal fungi present but no rhizobia 7
M+R+ effects of rhizobia and m ...... JA17 7 weeks roots mycorrhizal fungi and rhizobia present 8
Control Transcriptomic response t ...... wild type A17 5 days Root Hairs control 0h 2
NodFactor_20h Transcriptomic response t ...... wild type A17 5 days Root Hairs Nod Factor 20h 2
NodFactor_4h Transcriptomic response t ...... wild type A17 5 days Root Hairs Nod Factor 4h 2
BAP3hr Hormone treatment A17 3 days after germination root Cytokinin - 6-Benzylaminopurine;
Medicago seedlings Roots 		100 nM 6-Benzylaminopurine 3 hr 3
Control3hr Hormone treatment A17 3 days after germination root DMSO Control;
Medicago seedlings Roots 		DMSO Control 3 hr 3
IAA3hr Hormone treatment A17 3 days after germination root Auxin - Indole-3-acetic acid;
Medicago seedlings Roots 		1 uM Indole-3-acetic acid 3 hr 3
MtSm-RbmL-IZ Nodule Development 2 A17 22 days after germination + 15 days post inoculation root nodule ammonium nitrate supplemented;
Inoculation with the S. meliloti 2011 strain GMI11495 (4 days in N free prior to inoculation) 		10 m MNH4NO3 18 days;
22 days 		3
MtSm-RbmL-ZI Nodule Development 2 A17 22 days after germination + 15 days post inoculation root nodule ammonium nitrate supplemented;
Inoculation with the S. meliloti 2011 strain GMI11495 (4 days in N free prior to inoculation) 		10 m MNH4NO3 18 days;
22 days 		3
MtSm-RbmL-ZIId Nodule Development 2 A17 22 days after germination + 15 days post inoculation root nodule ammonium nitrate supplemented;
Inoculation with the S. meliloti 2011 strain GMI11495 (4 days in N free prior to inoculation) 		10 m MNH4NO3 18 days;
22 days 		3
MtSm-RbmL-ZIII Nodule Development 2 A17 22 days after germination + 15 days post inoculation root nodule ammonium nitrate supplemented;
Inoculation with the S. meliloti 2011 strain GMI11495 (4 days in N free prior to inoculation) 		10 m MNH4NO3 18 days;
22 days 		3
MtSm-RbmL-ZIIp Nodule Development 2 A17 22 days after germination + 15 days post inoculation root nodule ammonium nitrate supplemented;
Inoculation with the S. meliloti 2011 strain GMI11495 (4 days in N free prior to inoculation) 		10 m MNH4NO3 18 days;
22 days 		3