LegumeIP V3: From Models to Crops - An Integrative Gene Discovery Platform for Translational Genomics in Legumes

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Name Experiment Genotype Mutation Stage Tissue/organ Treatment Level Time replicates
A17_LCO_WT- CK dependent Rhizobium LC ...... A17 wild type genotype Susceptible zone E-MTAB-3007:A17 LCO WT002- 3h 3
A17_LCO_WT+ CK dependent Rhizobium LC ...... A17 wild type genotype Susceptible zone E-MTAB-3007:A17 LCO WT002+ 3h 3
A17_noLCO_WT- CK dependent Rhizobium LC ...... A17 wild type genotype Susceptible zone E-MTAB-3007:A17 noLCO WT003- 3h 3
A17_noLCO_WT+ CK dependent Rhizobium LC ...... A17 wild type genotype Susceptible zone E-MTAB-3007:A17 noLCO WT001+ 3h 3
Mtcre1_LCO_WT- CK dependent Rhizobium LC ...... A17 Mtcre1 mutant Susceptible zone E-MTAB-3007:Mtcre1 LCO WT003- 3h 3
Mtcre1_LCO_WT+ CK dependent Rhizobium LC ...... A17 Mtcre1 mutant Susceptible zone E-MTAB-3007:Mtcre1 LCO WT003+ 3h 3
Mtcre1_noLCO_WT- CK dependent Rhizobium LC ...... A17 Mtcre1 mutant Susceptible zone E-MTAB-3007:Mtcre1 noLCO WT001- 3h 3
Mtcre1_noLCO_WT+ CK dependent Rhizobium LC ...... A17 Mtcre1 mutant Susceptible zone E-MTAB-3007:Mtcre1 noLCO WT002+ 3h 3
A17_Mock_2dpi A17 and the ethylene inse ...... A17 root Mock 2dpi 3
A17_Mock_7dpi A17 and the ethylene inse ...... A17 root Mock 7dpi 3
A17_R.solani_inoculated_2dpi A17 and the ethylene inse ...... A17 root R.solani inoculated 2dpi 3
A17_R.solani_inoculated_7dpi A17 and the ethylene inse ...... A17 root R.solani inoculated 7dpi 3
skl_Mock_2dpi A17 and the ethylene inse ...... sickle mutant root Mock 2dpi 3
skl_Mock_7dpi A17 and the ethylene inse ...... sickle mutant root Mock 7dpi 3
skl_R.solani_inoculated_2dpi A17 and the ethylene inse ...... sickle mutant root R.solani inoculated 2dpi 3
skl_R.solani_inoculated_7dpi A17 and the ethylene inse ...... sickle mutant root R.solani inoculated 7dpi 3
BAP3hr Hormone treatment A17 3 days after germination root Cytokinin - 6-Benzylaminopurine;
Medicago seedlings Roots
100 nM 6-Benzylaminopurine 3 hr 3
Control3hr Hormone treatment A17 3 days after germination root DMSO Control;
Medicago seedlings Roots
DMSO Control 3 hr 3
IAA3hr Hormone treatment A17 3 days after germination root Auxin - Indole-3-acetic acid;
Medicago seedlings Roots
1 uM Indole-3-acetic acid 3 hr 3
Mt.nodule.T0 Transcriptomic changes in ...... Jemalong A17 8-week-old root nodule Inoculated with S. meliloti;
well-watered control nodules (T0)
2
Mt.nodule.T2 Transcriptomic changes in ...... Jemalong A17 8-week-old root nodule Inoculated with S. meliloti;
drought for two (T2) days
2
Mt.nodule.T4 Transcriptomic changes in ...... Jemalong A17 8-week-old root nodule Inoculated with S. meliloti;
drought for four (T4) days
2
Mt.root.T0 Transcriptomic changes in ...... Jemalong A17 8-week-old root Medicago truncatula non-inoculated roots;
well-watered control nodules (T0)
2
SUNN_Control_Root Symbiotic Interactions 2 A17 SUNN 18 days after planting root Whole root from un-colonized A17 sunn mutant Sterile soil At planting 3
SUNN_Control_Shoot Symbiotic Interactions 2 A17 SUNN 18 days after planting shoot Whole shoot from un-colonized A17 sunn mutant Sterile soil At planting 3
SUNN_Mycorrhiza_Root Symbiotic Interactions 2 A17 SUNN 18 days after planting root Whole root from mycorrhiza colonized A17 sunn mutant 10 % (w/w) soil inoculum At planting 3
SUNN_Mycorrhiza_Shoot Symbiotic Interactions 2 A17 SUNN 18 days after planting shoot Whole shoot from mycorrhiza colonized A17 sunn mutant 10 % (w/w) soil inoculum At planting 3
Wildtype_Control_Root Symbiotic Interactions 2 A17 None 18 days after planting root Whole root from un-colonized A17 wild type Sterile soil At planting 3
Wildtype_Control_Shoot Symbiotic Interactions 2 A17 None 18 days after planting shoot Whole shoot from un-colonized A17 wild type Sterile soil At planting 3
Wildtype_Mycorrhiza_Root Symbiotic Interactions 2 A17 None 18 days after planting root Whole root from mycorrhiza colonized A17 wild type 10 % (w/w) soil inoculum At planting 3